Evaluation of the efficacy of camel milk lactoferrin nanoliposome against colorectal cancer

Document Type : Original Article

Authors

1 Dairy Science Department, Faculty of Agriculture, Al-Azhar University, Cairo, Egypt.gypt

2 Dairy Science Department, Faculty of Agriculture, Al-Azhar University, Cairo, Egypt.

3 Dairy Res. Dept., Food Technology Research Institute, Agriculture Research Center, Giza, Egypt.

4 National Institute of Laser Enhanced Sciences (NILES). Cairo University, Giza, Egypt.

5 Faculty of Agriculture, Damanhour University, Damanhour, Egypt.

Abstract

Colorectal cancer (CRC) is a significant cause of death and cancer-related mortality worldwide. Lactoferrin (LF) is a promising molecule that was found to inhibit the growth of cancerous tumor.  This study explores the effect of camel milk lactoferrin nanoliposome (LFnl) on colorectal cancer (HTC-116 cells) as a potential therapeutic agent against colorectal cancer. The total purified camel milk LF concentration was 200 mg/l. Film hydration technique was used to produce LFnl. Advanced characterization revealed distinct nanoliposome shapes with a clear two-layered structure. The particle size ranged between 28.3 – 139 nm, and the zeta potential value was -22.6 mV. Exceptional encapsulation efficiency of 99.32% was achieved. The examination of HTC-116 cells and normal cells WI-38 elucidated an IC50 value of 40.5±0.9 µg/ml. while, the cytotoxicity assessments conducted on WI-38 cells exhibited a CC50 value of 43.5±0.3 µg/ml. This result indicated that LFnl should be used carefully when treated colorectal cancer.

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Journal of Plant and Food Sciences
Volume 1, Issue 2
December 2023
Pages 43-55

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